Assessment of Anti-SARS-CoV-2 antibody levels among university students vaccinated with different COVID-19 primary and booster doses - fall 2021, Wisconsin.

BACKGROUND: University students commonly received COVID-19 vaccinations before returning to U.S. campuses in the Fall of 2021. Given likely immunologic variation among students based on differences in type of primary series and/or booster dose vaccine received, we conducted serologic investigations in September and December 2021 on a large university campus in Wisconsin to assess anti-SARS-CoV-2 antibody levels. METHODS: We collected blood samples, demographic information, and COVID-19 illness and vaccination history from a convenience sample of students. Sera were analyzed for both anti-spike (anti-S) and anti-nucleocapsid (anti-N) antibody levels using World Health Organization standardized binding antibody units per milliliter (BAU/mL). Levels were compared across categorical primary COVID-19 vaccine series received and binary COVID-19 mRNA booster status. The association between anti-S levels and time since most recent vaccination dose was estimated by mixed-effects linear regression. RESULTS: In total, 356 students participated, of whom 219 (61.5%) had received a primary vaccine series of Pfizer-BioNTech or Moderna mRNA vaccines and 85 (23.9%) had received vaccines from Sinovac or Sinopharm. Median anti-S levels were significantly higher for mRNA primary vaccine series recipients (2.90 and 2.86 log [BAU/mL], respectively), compared with those who received Sinopharm or Sinovac vaccines (1.63 and 1.95 log [BAU/mL], respectively). Sinopharm and Sinovac vaccine recipients were associated with a significantly faster anti-S decline over time, compared with mRNA vaccine recipients (P <.001). By December, 48/172 (27.9%) participants reported receiving an mRNA COVID-19 vaccine booster, which reduced the anti-S antibody discrepancies between primary series vaccine types. CONCLUSIONS: Our work supports the benefit of heterologous boosting against COVID-19. COVID-19 mRNA vaccine booster doses were associated with increases in anti-SARS-CoV-2 antibody levels; following an mRNA booster dose, students with both mRNA and non-mRNA primary series receipt were associated with comparable levels of anti-S IgG.

Examining racial microaggressions and alcohol use among marginalized populations at a predominately white institution.

Objective: Microaggressions are brief verbal, behavioral, or environmental indignities toward people of color. Methods: Cross-sectional study examining the association between demographics, alcohol culture, and witnessing or experiencing microaggressions among undergraduate students. Analysis based on the "Color of drinking" study data collected at University of Wisconsin-Madison, WI between November 2017 and January 2018. Results: African-American/Black students significantly witnessed and experienced more microaggressions than other students, with a Relative Risk Ratio (RRR) of 9.5 (CI 95%: 4.7-19.1) and a RRR of 7 (CI 95%: 3.4-14.3). For students of color, experiencing microaggressions was associated with considering leaving (RRR = 3.5, CI 95%: 2-6.2). Additionally, the witnessing and experiencing of microaggressions appears to be associated with the alcohol culture on campus. Conclusions: African-American/Black was the racial group that witnessed and suffered more microaggressions. The percentage of students witnessing microaggressions increased with the year in school. Alcohol use in the last 30 days, feeling impacted by other's use of alcohol, and avoiding certain areas due to alcohol consumption were associated with experiencing microaggressions among students of color.

Interventions to Disrupt Coronavirus Disease Transmission at a University, Wisconsin, USA, August-October 2020.

University settings have demonstrated potential for coronavirus disease (COVID-19) outbreaks; they combine congregate living, substantial social activity, and a young population predisposed to mild illness. Using genomic and epidemiologic data, we describe a COVID-19 outbreak at the University of Wisconsin-Madison, Madison, Wisconsin, USA. During August-October 2020, a total of 3,485 students, including 856/6,162 students living in dormitories, tested positive. Case counts began rising during move-in week, August 25-31, 2020, then rose rapidly during September 1-11, 2020. The university initiated multiple prevention efforts, including quarantining 2 dormitories; a subsequent decline in cases was observed. Genomic surveillance of cases from Dane County, in which the university is located, did not find evidence of transmission from a large cluster of cases in the 2 quarantined dorms during the outbreak. Coordinated implementation of prevention measures can reduce COVID-19 spread in university settings and may limit spillover to the surrounding community.

Dating the Noceto Vasca Votiva, a unique wooden structure of the 15th century BCE, and the timing of a major societal change in the Bronze Age of northern Italy.

The Noceto 'Vasca Votiva' (votive tank), discovered in excavations on a terrace at the southern edge of the Po Plain, northern Italy, is a unique well-preserved wooden (primarily oak) structure dated to the advanced through late Middle Bronze Age (~1600-1300 BCE). This complex monument, comprising two super-imposed tanks, is generally linked with an important but uncertain ritual role involving water. The context provides extraordinary preservation of both wooden, other organic, and cultural finds. The key question until now, hindering further interpretation of this remarkable structure, has been the precise date of the tanks. Initial work pointed to use of the two tanks over about a century. Using dendrochronology and radiocarbon 'wiggle-matching' we report near-absolute construction dates for both of the tanks. The lower (older) tank is dated ~1444±4 BCE and the upper (more recent) tank is dated 12 years later at ~1432±4 BCE. This dating of the construction of the Noceto tanks in the 3rd quarter of the 15th century BCE further enables us to reassess the overall period of activity of this wooden complex and its association with a major period of societal change in the Bronze Age of northern Italy starting in the later 15th century BCE.

Mediterranean radiocarbon offsets and calendar dates for prehistory.

A single Northern Hemisphere calibration curve has formed the basis of radiocarbon dating in Europe and the Mediterranean for five decades, setting the time frame for prehistory. However, as measurement precision increases, there is mounting evidence for some small but substantive regional (partly growing season) offsets in same-year radiocarbon levels. Controlling for interlaboratory variation, we compare radiocarbon data from Europe and the Mediterranean in the second to earlier first millennia BCE. Consistent with recent findings in the second millennium CE, these data suggest that some small, but critical, periods of variation for Mediterranean radiocarbon levels exist, especially associated with major reversals or plateaus in the atmospheric radiocarbon record. At high precision, these variations potentially affect calendar dates for prehistory by up to a few decades, including, for example, Egyptian history and the much-debated Thera/Santorini volcanic eruption.

Radiocarbon re-dating of contact-era Iroquoian history in northeastern North America.

A time frame for late Iroquoian prehistory is firmly established on the basis of the presence/absence of European trade goods and other archeological indicators. However, independent dating evidence is lacking. We use 86 radiocarbon measurements to test and (re)define existing chronological understanding. Warminster, often associated with Cahiagué visited by S. de Champlain in 1615-1616 CE, yields a compatible radiocarbon-based age. However, a well-known late prehistoric site sequence in southern Ontario, Draper-Spang-Mantle, usually dated ~1450-1550, yields much later radiocarbon-based dates of ~1530-1615. The revised time frame dramatically rewrites 16th-century contact-era history in this region. Key processes of violent conflict, community coalescence, and the introduction of European goods all happened much later and more rapidly than previously assumed. Our results suggest the need to reconsider current understandings of contact-era dynamics across northeastern North America.

Fluctuating radiocarbon offsets observed in the southern Levant and implications for archaeological chronology debates.

Considerable work has gone into developing high-precision radiocarbon (14C) chronologies for the southern Levant region during the Late Bronze to Iron Age/early Biblical periods (∼1200-600 BC), but there has been little consideration whether the current standard Northern Hemisphere 14C calibration curve (IntCal13) is appropriate for this region. We measured 14C ages of calendar-dated tree rings from AD 1610 to 1940 from southern Jordan to investigate contemporary 14C levels and to compare these with IntCal13. Our data reveal an average offset of ∼19 14C years, but, more interestingly, this offset seems to vary in importance through time. While relatively small, such an offset has substantial relevance to high-resolution 14C chronologies for the southern Levant, both archaeological and paleoenvironmental. For example, reconsidering two published studies, we find differences, on average, of 60% between the 95.4% probability ranges determined from IntCal13 versus those approximately allowing for the observed offset pattern. Such differences affect, and even potentially undermine, several current archaeological and historical positions and controversies.

Integrated Tree-Ring-Radiocarbon High-Resolution Timeframe to Resolve Earlier Second Millennium BCE Mesopotamian Chronology.

500 years of ancient Near Eastern history from the earlier second millennium BCE, including such pivotal figures as Hammurabi of Babylon, Samsi-Adad I (who conquered Assur) and Zimrilim of Mari, has long floated in calendar time subject to rival chronological schemes up to 150+ years apart. Texts preserved on clay tablets provide much information, including some astronomical references, but despite 100+ years of scholarly effort, chronological resolution has proved impossible. Documents linked with specific Assyrian officials and rulers have been found and associated with archaeological wood samples at Kültepe and Acemhöyük in Turkey, and offer the potential to resolve this long-running problem. Here we show that previous work using tree-ring dating to place these timbers in absolute time has fundamental problems with key dendrochronological crossdates due to small sample numbers in overlapping years and insufficient critical assessment. To address, we have integrated secure dendrochronological sequences directly with radiocarbon (14C) measurements to achieve tightly resolved absolute (calendar) chronological associations and identify the secure links of this tree-ring chronology with the archaeological-historical evidence. The revised tree-ring-sequenced 14C time-series for Kültepe and Acemhöyük is compatible only with the so-called Middle Chronology and not with the rival High, Low or New Chronologies. This finding provides a robust resolution to a century of uncertainty in Mesopotamian chronology and scholarship, and a secure basis for construction of a coherent timeframe and history across the Near East and East Mediterranean in the earlier second millennium BCE. Our re-dating also affects an unusual tree-ring growth anomaly in wood from Porsuk, Turkey, previously tentatively associated with the Minoan eruption of the Santorini volcano. This tree-ring growth anomaly is now directly dated ~1681-1673 BCE (68.2% highest posterior density range), ~20 years earlier than previous assessments, indicating that it likely has no association with the subsequent Santorini volcanic eruption.

Old World megadroughts and pluvials during the Common Era.

Climate model projections suggest widespread drying in the Mediterranean Basin and wetting in Fennoscandia in the coming decades largely as a consequence of greenhouse gas forcing of climate. To place these and other "Old World" climate projections into historical perspective based on more complete estimates of natural hydroclimatic variability, we have developed the "Old World Drought Atlas" (OWDA), a set of year-to-year maps of tree-ring reconstructed summer wetness and dryness over Europe and the Mediterranean Basin during the Common Era. The OWDA matches historical accounts of severe drought and wetness with a spatial completeness not previously available. In addition, megadroughts reconstructed over north-central Europe in the 11th and mid-15th centuries reinforce other evidence from North America and Asia that droughts were more severe, extensive, and prolonged over Northern Hemisphere land areas before the 20th century, with an inadequate understanding of their causes. The OWDA provides new data to determine the causes of Old World drought and wetness and attribute past climate variability to forced and/or internal variability.